L1900

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

• Synonym(s): Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified
• UNSPSC Code: 41106200
• NACRES: NA.72

Properties

• Quality Level: 200
• form: powder
• technique(s): microbiological culture: suitable
• application(s): agriculture; microbiology

Description

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins.[1] Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.[2]

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable