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L1900

Sigma-Aldrich

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

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Synonym(s):
Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified
NACRES:
NA.72

Quality Level

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

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vibrant-m

L2025

Luria Agar Base, Miller

vibrant-m

L7275

LB Broth (Lennox)

vibrant-m

67454

Bolton Broth, Base

form

powder

form

powder

form

tablet

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

agriculture
microbiology

application(s)

agriculture
microbiology

application(s)

food and beverages
microbiology

application(s)

clinical testing
food and beverages
pathogen testing
sample preparation, microbiology

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26.0, 20-28 (2003)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26.0, 20-28 (2003)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)

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