L2020

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

• Synonym(s): Laminin
• CAS Number: 114956-81-9
• MDL number: MFCD00081739
• NACRES: NA.75

Properties

• biological source: mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
• Quality Level: 300
• product line: BioReagent
• form: aqueous solution
• mol wt: A subunit 400 kDa; B1 subunit 210 kDa; B2 subunit 200 kDa
• packaging: pkg of 1 mg
• concentration: 1-2 mg/mL in Tris-buffered saline
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑2 μg/cm²
• UniProt accession no.: P02468; P02469; P19137; Q61092
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Lama1(16772) , Lamb1(16777) , Lamc1(226519) , Lamc2(16782)

Description

Application

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:

• insulin-producing cell (IPC) differentiation.
• Infection inhibition assays.
• Proliferation Assay.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Caution

It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves