Documents

L2045

Sigma-Aldrich

Anti-LRP6 (C-terminal region) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Low-density lipoprotein receptor-related protein 6, Anti-ADCAD2
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~180 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

application(s)

immunoprecipitation (IP): 10-20 μg using lysate of HEK-293T cells expressing human LRP6
western blot: 1-2 μg/mL using lysate of HEK-293T cells expressing human LRP6

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LRP6(4040)
mouse ... Lrp6(16974)

General description

Low-density lipoprotein receptor-related protein-6 (LRP6) is expressed primarily in the central nervous system. This protein is made up of three subdomains in the extracellular domain, such as epidermal growth factor (EGF)-like domain, YWTD-type β-propeller domain, and low-density lipoprotein receptor (LDLR) type A (LA) domain. The LRP6 gene is located on the human chromosome at 12p13.2.

Specificity

Anti-LRP6 (C-terminal region) specifically recognizes human LRP6.

Application

Anti-LRP6 (C-terminal region) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Low-density lipoprotein receptor-related protein-6 (LRP6) is essential for proper brain development. The LRP6 protein forms a Wnt-Frizzled (Fz)-LRP6 complex that recruits axin to the plasma membrane resulting in the inhibition of βcatenin phosphorylation and degradation, and activation of T-cell factor (TCF)/lymphoid enhancer-binding factor-1 (LEF-1)-dependent transcription. Deletion of the LRP6 gene results in severe brain abnormalities. LRP6 also plays a central role in human disease. Mutations in the LRP6 gene are associated with coronary heart disease and osteoporosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

1

Severe defects in dorsal thalamic development in low-density lipoprotein receptor-related protein-6 mutants
Zhou C J, et al.
The Journal of Neuroscience, 24(35), 7632-7639 (2004)
LRP6 mutation in a family with early coronary disease and metabolic risk factors
Mani A, et al.
Science (New York, N.Y.), 315(5816), 1278-1282 (2007)
A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation
Zeng X, et al.
Nature, 438(7069), 873-877 (2005)
Adrián Mariño-Enríquez et al.
Human pathology, 39(12), 1844-1848 (2008-07-29)
Congenital mesenchymal tumors are diagnostically challenging as they are rare and may feature overlapping patterns between several benign, low-grade, and tumors of intermediate malignancy, including myofibromatosis, myofibroma/hemangiopericytoma, congenital fibrosarcoma, and inflammatory myofibroblastic tumor. Their immunophenotype is either silent or minimally...
Zhihong Cheng et al.
Nature structural & molecular biology, 18(11), 1204-1210 (2011-10-11)
Low-density-lipoprotein (LDL) receptor-related proteins 5 and 6 (LRP5/6) are Wnt co-receptors essential for Wnt/β-catenin signaling. Dickkopf 1 (DKK1) inhibits Wnt signaling by interacting with the extracellular domains of LRP5/6 and is a drug target for multiple diseases. Here we present...
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