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L2145

L-Leucine-7-amido-4-methylcoumarin hydrochloride

fluorogenic, ≥98% (TLC), powder

Synonym(s):

H-L-Leu-AMC HCl

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5 mg
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$122.00
25 mg
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$399.00
100 mg
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$1,200.00

About This Item

Empirical Formula (Hill Notation):
C16H20N2O3 · HCl
CAS Number:
62480-44-8
Molecular Weight:
324.80
UNSPSC Code:
12352204
PubChem Substance ID:
24896303
NACRES:
NA.32
MDL number:
MFCD00043262

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Product Name

L-Leucine-7-amido-4-methylcoumarin hydrochloride,

assay

≥98% (TLC)

Quality Level

200

form

powder

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 327 nm; λem 349 nm (pH 8.0), λex 380 nm; λem 440 nm (Reaction product)

storage temp.

−20°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1

InChI key

VCRXITKKWBOQRZ-ZOWNYOTGSA-N

General description

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) is a fluorogenic peptidyl substrate for leucine aminopeptidase, an extracellular enzyme found abundantly in natural aquatic systems. This enzyme is one of the various enzymes used by bacteria for protein hydrolysis.

Application

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) as a substrate analog to determine leucine aminopeptidase activity
L-Leucine-7-amido-4-methylcoumarin hydrochloride has been used:
  • as a substrate in fluorescence-based soil assays
  • to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes[1]

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This Item
A3401B4153164545
L-Leucine-7-amido-4-methylcoumarin hydrochloride

Sigma-Aldrich

L2145

L-Leucine-7-amido-4-methylcoumarin hydrochloride

Ala-Ala-Phe-7-amido-4-methylcoumarin protease substrate

Sigma-Aldrich

A3401

Ala-Ala-Phe-7-amido-4-methylcoumarin

Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin hydrochloride ~95%

Sigma-Aldrich

B4153

Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin hydrochloride

7-Amino-4-methylcoumarin Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.

Sigma-Aldrich

164545

7-Amino-4-methylcoumarin

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

~95%

assay

≥98% (HPLC)

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

solubility

ethanol: 20 mg/mL, clear, colorless to light yellow

solubility

methanol: 50 mg/mL, clear, colorless

solubility

DMSO: 10 mg/mL, acetone: 10 mg/mL, DMF: soluble

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

powder

form

powder

form

solid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

fluorescence

λex 327 nm; λem 349 nm (pH 8.0), λex 380 nm; λem 440 nm (Reaction product)

fluorescence

-

fluorescence

-

fluorescence

λex 365-380 nm, λem 430-460 nm

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM8046
BCCL8090
BCCK0769
BCCG2323
BCCD7189

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Jisook Lee et al.
ChemMedChem, 16(1), 234-249 (2020-09-19)
Aminopeptidase N (APN/CD13) is a zinc-dependent ubiquitous transmembrane ectoenzyme that is widely present in different types of cells. APN is one of the most extensively studied metalloaminopeptidases as an anti-cancer target due to its significant role in the regulation of
Enzyme activity responses to nutrient loading in subtropical wetlands
Penton CR and Newman S
Biogeochemistry, 84, 83-98 (2007)
Vincent Tardy et al.
Journal of hazardous materials, 411, 125121-125121 (2021-04-17)
Pharmaceutical industry effluents are complex and highly variable in time. Assessing the efficiency of a pharmaceutical industry wastewater treatment plant (WWTP) and the resulting decrease in effluent toxicity and ecological risk is thus not straightforward. We set up an original
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Global Trade Item Number

SKUGTIN
L2145-5MG04061832908816
L2145-100MG04061833949856
L2145-25MG04061833949863

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