L2420

Sigma-Aldrich

Monoclonal Anti-LRP1 antibody produced in mouse

~1.0 mg/mL, clone LRP1-11, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-Lipoprotein receptor-related protein, Anti-Apolipoprotein receptor, Anti-A2MR, Anti-LRP, Anti-Low density lipoprotein receptor-related protein 1, Anti-APOER, Anti-APR, Anti-Apolipoprotein E receptor, Anti-CD91
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LRP1-11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~500 kDa

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

application(s)

indirect immunofluorescence: suitable
western blot: 1-2 μg/mL

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LRP1(4035)
mouse ... Lrp1(16971), Lrp1(299858)

General description

Monoclonal Anti-LRP1 (mouse IgG1 isotype) is derived from the hybridoma LRP1-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human LRP1, conjugated to KLH. LDL receptor family. Low-density lipoprotein (LDL) receptor related protein 1 (LRP1) gene is mapped to human chromosome 12q13.3. LRP1 protein, belongs to the LDL receptor family. LRP1, a single-chain 600 kDa type I transmembrane glycosylated precursor protein, is produced in the endoplasmic reticulum. This protein is proteolytically cleaved in the Golgi complex into two subunits including 85-kDa membrane-bound C-terminal membrane spanning subunit and a noncovalently attached 515-kDa N-terminal subunit.

Specificity

Monoclonal Anti-LRP1 recognizes human, rat, and mouse LRP1.

Application

Monoclonal Anti-LRP1 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay
  • immunofluorescence

Biochem/physiol Actions

Low density lipoprotein receptor related protein 1 (LRP1) plays an essential role in early stages of embryonic development. It aids in lipoprotein metabolism, proteinase regulation, activation of lysosomal enzymes and cellular entry of viruses and toxins. Additionally, It is also implicated in cell signaling pathways, cell migration, and neurodevelopment. LRP1 interacts with at least 30 different ligands including lipoproteins, proteinases, proteinase-inhibitor complexes, extra cellular matrix (ECM) proteins and various intracellular proteins. Furthermore, LDL receptor related protein 1 also has an ability to associate with bacterial toxins and viruses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Deficits in receptor-mediated endocytosis and recycling in cells from mice with Gpr107 locus disruption
Zhou G, et al.
Journal of Cell Science, 127(18), 3916-3927 (2014)
Takahisa Kanekiyo et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(49), 19276-19283 (2013-12-07)
Alzheimer's disease (AD) is the most prevalent form of dementia in the elderly population. Accumulation, aggregation, and deposition of amyloid-β (Aβ) peptides generated through proteolytic cleavage of amyloid precursor protein (APP) are likely initiating events in the pathogenesis of AD....
Kazuhiro Yamamoto et al.
The Journal of biological chemistry, 289(10), 6462-6474 (2014-01-30)
Degradation of the cartilage proteoglycan aggrecan is an early event in the development of osteoarthritis, and a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4) and ADAMTS-5 are considered to be the major aggrecan-degrading enzymes. We have recently found that ADAMTS-5...
Inflammatory mediators promote production of shed LRP1/CD91, which regulates cell signaling and cytokine expression by macrophages
Gorovoy M, et al.
Journal of Leukocyte Biology, 88(4), 769-778 (2010)
Beyond endocytosis: LRP function in cell migration, proliferation and vascular permeability
Lillis AP, et al.
Journal of Thrombosis and Haemostasis, 3(8), 1884-1893 (2005)

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