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L2524

Sigma-Aldrich

Lyticase from Arthrobacter luteus

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

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Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
CAS Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥2,000 units/mg protein

composition

Protein, ≥20% biuret

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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1 of 4

This Item
L4025A6814SAE0098
specific activity

≥2,000 units/mg protein

specific activity

≥200 units/mg solid

specific activity

≥400 units/mg protein (Lowry)

specific activity

≥2000 units/mg protein

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

-

composition

Protein, ≥20% biuret

composition

-

composition

-

composition

-

Application

Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.

Biochem/physiol Actions

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains β-(1→3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Physical form

Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

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