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  • L2542
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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Synonym(s):
Luria Broth Miller
NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

sterile; 0.2 μm filtered

form

liquid

pH

6.5-7.2

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Packaging

500 mL

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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