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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

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Synonym(s):
Luria Broth Miller, Luria-Bertani broth, Miller
NACRES:
NA.85

grade

for molecular biology

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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L3522L3147L3272
LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

liquid

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

6.5-7.2

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(4% solution)

pH

6.8-7.2(3.05% solution)

application(s)

microbiology

application(s)

food and beverages

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Articles

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocols

General protocols for growth of competent cells in microbial medium.

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