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L2646

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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Size/SKUAvailabilityPrice
10 mg
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$79.10
25 mg
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$125.00
100 mg
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$380.00

About This Item

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder

$79.10

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Quality Segment

300

form

lyophilized powder

potency

<40 μg per mL agglutination activity

composition

Protein, 50-70% biuret

impurities

salt, free

storage temp.

2-8°C

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

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This Item
L2769L8902L5380
Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder

Sigma-Aldrich

L2646

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L8902

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Pisum sativum (pea) lyophilized powder

Sigma-Aldrich

L5380

Lectin from Pisum sativum (pea)

description

Phytohemagglutinin PHA-M, lyophilized powder

description

Leucoagglutinin PHA-L, lyophilized powder

description

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

description

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

potency

<40 μg per mL agglutination activity

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity

potency

<40 μg per mL agglutination activity

potency

<20 μg/mL per mL agglutination activity

composition

Protein, 50-70% biuret

composition

Protein, ≥60% biuret

composition

-

composition

Protein, ≥70% biuret

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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