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L2897

Sigma-Aldrich

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

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Synonym(s):
Lennox broth, Luria Bertani Agar
NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L7658L3272L2542
LB Broth (Lennox) EZMix™ powder microbial growth medium

Sigma-Aldrich

L7658

LB Broth (Lennox)

LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

form

powder

form

powder

form

powder

form

liquid

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

-

composition

Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

microbiology

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth with agar (Lennox) has been used for:

  • the transformation of competent E. coli cells
  • to cultivate Ecc-15 bacteria
  • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
  • transmission electron microscopy (TEM) and immunohistochemistry (IHC)
It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Easy scale-up using larger package sizes
  • Standard formulation

Preparation Note

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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MMYOMAG-74K-13

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Ultrastructural visualization of the Mesenchymal-to-Epithelial Transition during reprogramming of human fibroblasts to induced pluripotent stem cells
Hoffding, MK and Hyttel, P
Current Stem Cell Research & Therapy, 14.0, 39-53 (2015)
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Langmuir : the ACS journal of surfaces and colloids, 34(24), 7192-7200 (2018-05-22)
Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive
Structural analysis of three-dimensional human neural tissue derived from induced pluripotent stem cells
Brooks PT, et al.
Journal of Separation Science, 6.0, 337-337 (2016)
Shun Gen Huang et al.
BMC immunology, 14, 27-27 (2013-06-22)
Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible
Le Cong et al.
Methods in molecular biology (Clifton, N.J.), 1239, 197-217 (2014-11-20)
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that exists in a variety of microbes. It could be engineered to function in eukaryotic cells as a fast, low-cost, efficient, and scalable tool for manipulating

Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service