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L3001

Sigma-Aldrich

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

wheat (germ)

Quality Level

type

Type I

form

lyophilized powder

specific activity

5-15 units/mg solid

UniProt accession no.

storage temp.

2-8°C

Gene Information

wheat ... lipase(543006)

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General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:
  • to prepare enzyme solution for the biodegradability test
  • to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
  • to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Packaging

1, 5 g in poly bottle

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Quality

Contains acid phosphatase.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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