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L3022

Sigma-Aldrich

LB Broth (Lennox)

Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Synonym(s):
Lennox broth , Lennox broth, Luria Bertani Broth
NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

form

powder

composition

NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH

6.8-7.2(2% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
It was used for resuspension of T. pyriformis cell fractions in a study of Trimastix fractionation of cellular extracts.

Packaging

250 g in poly bottle
1 kg in poly bottle

Features and Benefits

Lennox LB powder provides:
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to liquid
  • Standard formulation

Preparation Note

1. Suspend 20 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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