This product has not been tested with synthetic substrate pNP-Palmitate in-house. Its activity on pNP-Palmitate has been cited in the following article that may be of interest as a reference: Int J Biol Macromol. 2023 Mar 1;230:123427.
L3126
Lipase from porcine pancreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Lipase from porcine pancreas
Synonym(s):
Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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| Size/SKU | Availability | Price |
|---|---|---|
25 g | Available to ship onJuly 15, 2026fromMILWAUKEE | $62.90 |
100 g | Available to ship onJuly 15, 2026fromMILWAUKEE | $82.00 |
500 g | Available to ship onJuly 15, 2026fromMILWAUKEE | $297.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
MDL number:
Specific activity:
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Biological source:
Porcine pancreas
biological source
Porcine pancreas
Quality Segment
type
Type II
form
lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
composition
protein, ≥20%
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
Application
Lipase from porcine pancreas has been used:
- for the analysis of fatty acid at the 2-position of triglycerides.
- to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
- as a component of dissolution media to study the dissolution profiles of theophylline.[1]
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Analysis Note
Crude (Steapsin). Contains amylase and protease activity.
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
1 of 1
This Item | |||
|---|---|---|---|
| description Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin) | description Type VI-S, ≥20,000 units/mg protein, lyophilized powder | description lyophilized powder, ≥40,000 units/mg protein | description Type VII, ≥700 unit/mg solid |
| specific activity ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin) | specific activity ≥20,000 units/mg protein | specific activity ≥40,000 units/mg protein | specific activity ≥700 unit/mg solid |
| biological source Porcine pancreas | biological source - | biological source fungus (Candida rugosa) | biological source (from Candida rugosa) |
| form lyophilized (cake) | form lyophilized powder | form lyophilized powder | form lyophilized |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| type Type II | type Type VI-S | type - | type Type VII |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can I use the synthetic substrate pNP-Palmitate to perform the activity assay, or does this enzyme only have activity with a natural substrate?
1 answer-
Helpful?
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How was the olive oil prepared for the activity determination? Was it emulsified? If yes, in which oil:water ratio? Was any emulsifier used?
1 answer-
This product contains 50% (v/v) olive oil (catalog number O1500).
1% sodium azide is added as a preservative and an emulsifier. The exact composition and method of
composition is considered proprietary and therefore will not be disclosed.Helpful?
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May I know the PDB information related to this lipase protein?
1 answer-
PDB information is not available for this product.
Helpful?
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I want to use this product (L3126) for preparing a solution to conduct in vitro degradation experiments on a particular material. Could you please provide detailed instructions on how to prepare the solution, including any recommendation?
1 answer-
Unfortunately, there is no application specific instructions available for this product. The material may be reconstituted in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA. This product is from porcine pancreas. Another product option, L3419 Lipase from Thermus thermophilus, is reconstituted in 50 mM Tris-HCl, pH 8.0. This material states that solutions are stable for up to 1 month at 2 - 8°C. Please see the link below to review the datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/504/l3419dat.pdfHelpful?
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How is a "microequivalent" of fatty acid quantified in the enzymatic activity unit definition for L3126?
1 answer-
A microequivalent is equivalent to 1 mmol/L. In this case, one unit of Lipase will hydrolyze 1.0 microequivalent (1 mmol/L) of fatty acid from olive oil in 1 hour at pH 7.7 at 37°C.
Helpful?
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