This product contains 50% (v/v) olive oil (catalog number O1500).
1% sodium azide is added as a preservative and an emulsifier. The exact composition and method of
composition is considered proprietary and therefore will not be disclosed.
L3126
Lipase from porcine pancreas
Lipase from porcine pancreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Synonym(s):
Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
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25 G
$55.76
100 G
$76.50
500 G
$277.00
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25 G
$55.76
100 G
$76.50
500 G
$277.00
About This Item
Recommended Products
biological source
Porcine pancreas
type
Type II
form
lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)
composition
protein, ≥20%
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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Related Categories
1 of 4
This Item | 62302 | 62310 | 62316 |
|---|---|---|---|
| biological source Porcine pancreas | biological source fungus (Candida rugosa) | biological source fungus (Rhizopus niveus) | biological source fungus (Candida rugosa) |
| specific activity ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin) | specific activity 15-25 U/mg | specific activity ≥1.5 U/mg | specific activity ≥2 U/mg |
| form lyophilized (cake) | form lyophilized solid, powder (fine) | form powder (fine) | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| type Type II | type - | type - | type - |
Application
Lipase from porcine pancreas has been used:
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Quality
Crude (Steapsin). Contains amylase and protease activity.
Unit Definition
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Analysis Note
Protein determined by biuret.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Customers Also Viewed
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How was the olive oil prepared for the activity determination? Was it emulsified? If yes, in which oil:water ratio? Was any emulsifier used?
1 answer-
Helpful?
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May I know the PDB information related to this lipase protein?
1 answer-
PDB information is not available for this product.
Helpful?
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I want to use this product (L3126) for preparing a solution to conduct in vitro degradation experiments on a particular material. Could you please provide detailed instructions on how to prepare the solution, including any recommendation?
1 answer-
Unfortunately, there is no application specific instructions available for this product. The material may be reconstituted in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA. This product is from porcine pancreas. Another product option, L3419 Lipase from Thermus thermophilus, is reconstituted in 50 mM Tris-HCl, pH 8.0. This material states that solutions are stable for up to 1 month at 2 - 8°C. Please see the link below to review the datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/504/l3419dat.pdfHelpful?
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How is a "microequivalent" of fatty acid quantified in the enzymatic activity unit definition for L3126?
1 answer-
A microequivalent is equivalent to 1 mmol/L. In this case, one unit of Lipase will hydrolyze 1.0 microequivalent (1 mmol/L) of fatty acid from olive oil in 1 hour at pH 7.7 at 37°C.
Helpful?
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