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L3147

Sigma-Aldrich

LB Broth with agar (Miller)

Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

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Synonym(s):
Luria Agar, Luria Bertani agar, Miller’s LB agar, lysogeny broth agar
eCl@ss:
42040102
NACRES:
NA.85

grade

for molecular biology

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(4% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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L3522L3272L2542
Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

form

powder

form

powder

form

powder

form

liquid

composition

Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

-

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

6.8-7.2(4% solution)

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(3.05% solution)

pH

6.5-7.2

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Miller′s LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Preparation Note

1. Suspend 40 g in 1L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Luria, S.E., and Burrous, J.W.
Journal of Bacteriology, 74, 461-476 (1955)

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Protocols

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General protocols for growth of competent cells in microbial medium.

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