L3147

Sigma-Aldrich

LB Broth with agar (Miller)

Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

Synonym(s):
Luria Agar, Miller’s LB agar
eCl@ss:
42040102
NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH range

6.8 - 7.2 (4% solution)

Featured Industry

Food and Beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.

Packaging

250 g in poly bottle
1 kg in poly bottle

Features and Benefits

Miller′s LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Preparation Note

1. Suspend 40 g in 1L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference among the LB - Miller products?

    LB - Miller is available in many types to suit your needs.  The different product formats include powder and liquid form.  The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Difco Manual, 239-239 (1998)
Multiple microbial activities for volatile organic compounds reduction by biofiltration
Civilini M.
Journal of the Air & Waste Management Association (1995), 56(7), 922-930 (2006)
Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water
McGuigan KG, et al.
Journal of Applied Microbiology, 101(2), 453-463 (2006)
Gang Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7718-7732 (2020-04-16)
Liver inflammation is a common extraintestinal manifestation in inflammatory bowel disease (IBD), yet, the mechanisms driving gut-liver axis inflammation remain poorly understood. IBD leads to a breakdown in the integrity of the intestinal barrier causing an increase in portal and...
Articles
The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.
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Protocols
General protocols for growth of competent cells in microbial medium.
Read More

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