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L3160

Sigma-Aldrich

Monoclonal Anti-Leptin antibody produced in mouse

clone LEP-13, ascites fluid

Synonym(s):

Anti-OB

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LEP-13, monoclonal

mol wt

antigen 16 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:1,000 using recombinant human leptin

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LEP(3952)

General description

Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. The receptors are present in brain, lungs, kidneys, liver and adipose tissue. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK. Deficiency of leptin may be a congenital condition and results in thyroid axis abnormalities.. Monoclonal Anti-Leptin reacts specifically with human leptin. A weak cross reactivity with mouse leptin is observed in radioimmunoassay.
Monoclonal Anti-Leptin (OB) (mouse IgG isotype) is derived from the LEP-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human leptin. Leptin (also known as OB, the product of the ob gene) is a 16 kDa, 146 amino acid residue, non-glycosylated polypeptide, secreted by the mature adipocyte. It is strongly expressed in white adipose tissue, and is absent or expressed at extremely low levels in other tissues. The human leptin molecule is translated as a 167 amino acid residue polypeptide, with the first 21 amino acid residues cleaved as a signal peptide.

Specificity

The antibody reacts specifically with human leptin. By RIA, weak cross-reactivity has been observed with recombinant mouse leptin.

Immunogen

recombinant human leptin.

Application

Monoclonal Anti-Leptin antibody produced in mouse has been used in western blotting. It may be used in radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Leptin is hypothesized to be a ′′satiety factor′′, because the absence of this factor was associated with hyperphagia and obesity. Leptin is involved in appetite regulation. Human obesity is often associated with increased blood leptin levels. Leptin acts through discrete receptors and distant targets to create a feedback loop for body weight regulation. With respect to the target of leptin action, the hypothalamus has received a great deal of attention. The leptin system has also been proposed to contribute to early hematopoiesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Bisphenol A induces leptin receptor expression, creating more binding sites for leptin, and activates the JAK/Stat, MAPK/ERK and PI3K/Akt signalling pathways in human ovarian cancer cell
Ptak A and Gregoraszczuk EL
Toxicology Letters, 210(3), 332-337 (2012)
Superactive human leptin antagonist reverses leptin-induced excessive progesterone and testosterone secretion in porcine ovarian follicles by blocking leptin receptors
Gregoraszczuk EL and Rak A
Journal of Physiology And Pharmacology, 66(3), 39-46 (2015)
Narrative review: the role of leptin in human physiology: emerging clinical applications
Kelesidis T, et al.
Annals of Internal Medicine, 152(2), 93-93 (2010)
Sami M Khan et al.
Yonsei medical journal, 53(4), 671-679 (2012-06-06)
Leptin, a peptide hormone secreted by adipocytes in proportion of the amount of energy stored in fat, plays a central role in regulating human energy homeostasis. In addition, leptin plays a significant permissive role in the physiological regulation of several
Michal Duliban et al.
International journal of molecular sciences, 21(10) (2020-05-28)
Although epidemiological studies from the last years report an increase in the incidences of Leydig cell tumors (previously thought to be a rare disease), the biochemical characteristics of that tumor important for understanding its etiology, diagnosis, and therapy still remains

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