LB Broth with agar (Luria low salt) (L3272) is generally suitable for microbiological culture and is nonselective, but there is no explicit confirmation in the provided documentation that it is suitable for Bacillus megaterium.
L3272
LB Broth with agar (Luria low salt)
Powder microbial growth medium
Synonym(s):
Luria Agar Base (Miller’s Modification)
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kg | Available to ship TODAYfromMILWAUKEE | $256.00 |
About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
$256.00
Available to ship TODAYDetails
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
Application
LB Broth with agar (Luria low salt) has been used:
- for culturing E. coli DH10B cells.
- in the isolation of diazotrophic bacteria.
- for transformation of competent E. coli strain Stbl3 for cloning. [1]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Luria′s low salt LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Preparation Note
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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This Item | |||
|---|---|---|---|
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability - | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| composition Agar, 15 g/L , Tryptone, 10 g/L , NaCl, 0.5 g/L , Yeast Extract, 5 g/L | composition NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
General protocols for growth of competent cells in microbial medium.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| L3272-1KG | 04061833950951 |
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Is it suitable for b. Megaterium culture ?
1 answer-
Helpful?
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What is the difference between the LB - Luria low salt products?
1 answer-
LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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