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L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

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Synonym(s):
Luria Agar Base (Miller’s Modification)

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.05% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L3397L3147L3522
Sigma-Aldrich

Sigma-Aldrich

L3522

LB Broth (Miller)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

composition

Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

pH

6.8-7.2(3.05% solution)

pH

6.8-7.2(1.55% solution)

pH

6.8-7.2(4% solution)

pH

6.8-7.2(2.5% solution)

General description

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

LB Broth with agar (Luria low salt) has been used:
  • for culturing E. coli DH10B cells.
  • in the isolation of diazotrophic bacteria.
  • for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Preparation Note

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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