L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

Synonym(s):
Luria Agar Base (Miller’s Modification)
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH range

6.8 - 7.2 (3.05% solution)

Featured Industry

Food and Beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

LB Broth with agar (Luria low salt) has been used for culturing E. coli DH10B cells and in the isolation of diazotrophic bacteria.

Features and Benefits

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Preparation Note

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference between the LB - Luria low salt products?

    LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth
Grobelak A, et al.
Ecological Engineering, 84, 22-28 (2015)
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
Difco Manual, 271-271 (1998)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes...
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Protocols
General protocols for growth of competent cells in microbial medium.
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