L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Synonym(s): Luria Broth (Miller’s Modification)
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• composition: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• pH: 6.8-7.2(1.55% solution)
• application(s): food and beverages
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low salt formulation.

Application

LB Broth (Luria low salt) has been used as a diluting agent for the preparation of dilution series of free levofloxacin (LVF) and LVF encircled by nanohydrogel (LVF-NHs).

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Packaging

250 g

1 kg

Features and Benefits

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Preparation Note

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable