Documents

L3414

Sigma-Aldrich

Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse

~2 mg/mL, clone 6E10, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-Cardiomyopathy, dilated, 1T, included, Anti-CMD1T, Anti-Lamina Associated Polypeptide 2, Anti-TMPO, Anti-Thymopoietin
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E10, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa (ζ isoform)
antigen 38 kDa (γ isoform, singlet or doublet)
antigen 42 kDa (δ isoform)
antigen 45 kDa (ε isoform)
antigen 50 kDa (β isoform, singlet or doublet)
antigen 76 kDa (α isoform)

species reactivity

human

concentration

~2 mg/mL

application(s)

immunocytochemistry: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured human chronic myelogenous leukemia K562 cells.

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TMPO(7112)

General description

Monoclonal Anti-LAP2 (TMPO) (mouse IgG1 isotype) is derived from the 6E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide (amino acids 29-50) common to the N-terminus of all TMPOs. Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is a type II integral protein of the inner nuclear envelope (NE). In mammalian cells, six alternatively spliced TMPO isoforms, designated α, β, β′, γ, δ, ε, and ζ have been isolated and characterized. LAP2/TMPO proteins is highly conserved in mammals and Xenopus. The expression of the various TMPO isoforms is ubiquitous, with higher levels in proliferative tissues. It has four domains: a hydrophilic C-terminus domain, a hydrophobic transmembrane domain, a NE targeting and lamina-binding domain, and a chromatin binding domain. TMPOβ/LAP2 contains two putative p34cdc2 kinase phosphorylation sites.

Immunogen

synthetic peptide corresponding to amino acids 29-50 common to the N-terminus of all human TMPOs (isoforms α, β, γ, δ, ε, and ζ).

Application

Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is putatively involved in functional nuclear architecture and cell cycle control. TMPOβ/LAP2 plays a key role in nuclear envelope (NE) disassembly and reassembly during mitosis and linking chromatin to the NE in interphase. TMPOβ/LAP2 binds lamin B1 and chromosomes in a phosphorylation dependent manner. TMPOβ/LAP2, either together with germ-cell-less (GCL) or alone, can repress the transcriptional activity of the elongation factor 2-dimerization partner (E2F-DP) heterodimer. TMPOα/LAP2 has a role in regulating the dynamics of the nuclear lamina and this interaction is required for nuclear growth after mitosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

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Certificate of Analysis
Certificate of Origin
Detergent-salt resistance of LAP2alpha
Dechat T, et al.
The Embo Journal, 17(16), 4887-4902 (1998)
Review: Lamina-Associated Polypeptide 2 Isoforms and Related Proteins in Cell Cycle-Dependent Nuclear Structure Dynamics
Dechat T, et al.
Journal of Structural Biology, 129(2), 335-345 (2000)
Goldberg, M., et al.
Gene Therapy, 4, 143-143 (1999)

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