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L3522

Sigma-Aldrich

LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Synonym(s):
Luria Broth, Miller’s LB Broth
NACRES:
NA.85

grade

for molecular biology

Quality Level

description

Miller′s Modification, 10 g/L NaCl

sterility

non-sterile

form

powder

composition

NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Features and Benefits

Miller′s LB powder provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to liquid
  • Standard formulation

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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