Documents
L3522
LB Broth (Miller)
Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture
Recommended Products
grade
for molecular biology
Quality Level
description
Miller′s Modification, 10 g/L NaCl
sterility
non-sterile
form
powder
composition
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
pH
6.8-7.2(2.5% solution)
application(s)
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
1 of 4
This Item | L2542 | L3272 | L3147 |
|---|---|---|---|
| sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility non-sterile |
| form powder | form liquid | form powder | form powder |
| composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition - | composition Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| pH 6.8-7.2(2.5% solution) | pH 6.5-7.2 | pH 6.8-7.2(3.05% solution) | pH 6.8-7.2(4% solution) |
| application(s) food and beverages | application(s) - | application(s) food and beverages | application(s) food and beverages |
General description
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Biochem/physiol Actions
Features and Benefits
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
- Standard formulation
Preparation Note
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
Reconstitution
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
General protocols for growth of competent cells in microbial medium.
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