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L3540

Supelco

Lowry Reagent

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Synonym(s):
Copper(II) tartrate Mix

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TP0300F9252MXQREAG48
vibrant-m

L3540

Lowry Reagent

vibrant-m

MXQREAG48

Milliflex® Quantum reagents

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.
Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

Reconstitution

Reconstitute to 40 mL with deionized water.

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Storage Class

4.1B - Flammable solid hazardous materials

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WGK 2

flash_point_f

Not applicable

flash_point_c

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PLoS ONE, 8(9), e76106-e76106 (2013)
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Molecular Systems Biology, 7(1), 563-563 (2011)
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
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Protocols

To standardize a procedure for the determination of protein by modified Lowry.

Related Content

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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