This product is not tested for purity. Only the enzymatic activity in U/mL is provided. The certificate of analysis contains the release specifications.
L3916
L-Lactic Dehydrogenase from bovine heart
1000 units/mL
Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
Specific activity:
1000 units/mL
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This Item | L1006 | L2625 | L1378 |
|---|---|---|---|
| specific activity 1000 units/mL | specific activity ≥400 units/mg protein | specific activity ≥500 units/mg protein | specific activity ≥600 units/mg protein |
| form solution | form buffered aqueous glycerol solution | form ammonium sulfate suspension | form ammonium sulfate suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Application
L-Lactic Dehydrogenase from bovine heart has been used:
- as a reference standard to measure the LDH (lactic dehydrogenase)[3]
- to quench protein-bound NADH in cells[4]
- to measure the lactate released by the cells in suspension[5]
For use in enzymatic determination of lactate or pyruvate.
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.[1]
Physical form
Suspension in 2.2 M ammonium sulfate
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocols
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
Related Content
Instructions
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