MilliporeSigma
All Photos(2)

L4021

Sigma-Aldrich

Prolactin human

recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
hPRL, LTH, Lactogenic Hormone, Luteotropic Hormone, PRL
CAS Number:
MDL number:

recombinant

expressed in E. coli

Quality Level

description

200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227).

product line

BioReagent

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.030-0.300 ng/mL EC50

mol wt

predicted mol wt ~24 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: 0.5000 mL, clear, colorless

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... PRL(5617)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRP9000S1763S4776
Prolactin human recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)

Sigma-Aldrich

L4021

Prolactin human

Prolactin human human, recombinant, expressed in HEK 293 cells

Sigma-Aldrich

SRP9000

Prolactin human

Somatostatin powder, BioReagent, suitable for cell culture

Sigma-Aldrich

S1763

Somatostatin

product line

BioReagent

product line

-

product line

BioReagent

product line

-

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥97% (HPLC)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

form

liquid

form

powder

form

lyophilized powder

potency

0.030-0.300 ng/mL EC50

potency

≤2 ng/mL Nb2-11 cells proliferation EC50

potency

-

potency

0.025-0.1 ng/mL cell proliferation using Nb2-11 cells per bioactivity ED50

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Application

Use in cell culture applications to study prolactin regulation of cell signaling and gene expression.

Biochem/physiol Actions

Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes, and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

Other Notes

Contains an N-terminal methionine not present in the natural product.

Physical form

Lyophilized from a solution in 10 mM phosphate and 50 mM sodium chloride, pH 8.0.

Reconstitution

It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Analysis Note

The biological activity is assayed by proliferation of the rat lymphoma cell line, Nb2-11.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Sodium selenite BioReagent, suitable for cell culture, ≥98%

Sigma-Aldrich

S5261

Sodium selenite

Hydrocortisone BioReagent, suitable for cell culture

Sigma-Aldrich

H0888

Hydrocortisone

Etienne Delgrange et al.
European journal of endocrinology, 170(1), 31-38 (2013-10-04)
To characterise distinctive clinical features of giant prolactinomas in women. A multicentre, retrospective case series and literature review. We collected data from 15 female patients with a pituitary tumour larger than 4 cm and prolactin levels above 1000 μg/l and
Julien Auffret et al.
American journal of physiology. Endocrinology and metabolism, 305(10), E1309-E1318 (2013-09-26)
Prolactin (PRL) and placental lactogens stimulate β-cell replication and insulin production in pancreatic islets and insulinoma cells through binding to the PRL receptor (PRLR). However, the contribution of PRLR signaling to β-cell ontogeny and function in perinatal life and the
Extrapituitary prolactin: distribution, regulation, functions, and clinical aspects.
N Ben-Jonathan et al.
Endocrine reviews, 17(6), 639-669 (1996-12-01)
Gerda de Kuijper et al.
Research in developmental disabilities, 34(9), 2799-2809 (2013-06-25)
Individuals with intellectual disability frequently use antipsychotics for many years. This may have detrimental health effects, including neurological symptoms and metabolic and hormonal dysregulation, the latter possibly affecting bone metabolism. There is large variability in the degree in which antipsychotic
Khalid Bajou et al.
Nature medicine, 20(7), 741-747 (2014-06-16)
The N-terminal fragment of prolactin (16K PRL) inhibits tumor growth by impairing angiogenesis, but the underlying mechanisms are unknown. Here, we found that 16K PRL binds the fibrinolytic inhibitor plasminogen activator inhibitor-1 (PAI-1), which is known to contextually promote tumor

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service