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L4277

Sigma-Aldrich

Lipase from Rhizomucor miehei

≥20,000 U/g

Synonym(s):
Palatase® 20,000L
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized

Quality Level

specific activity

≥20,000 U/g

storage temp.

2-8°C

General description

Rhizomucor miehei lipase is a carboxylesterase and belongs to the α/β-hydrolase family. It comprises a lid domain, hinge domain and a catalytic triad Ser?His?Asp/Glu. The three-dimensional structure is a α/β hydrolase fold which is very similar as that of esterase enzyme.

Application

Lipase from Rhizomuco miehei has been used:

  • in the partial digestion of triacylglycerols (TAG)
  • in the digestion of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR) present in castor oil
  • in the hydrolysis of linseed oil

Packaging

50, 250 mL in glass bottle

Biochem/physiol Actions

Lipase catalyzes the hydrolysis of monoacylglycerols and diacylglycerols and its activity is inhibited by divalent actions. Rhizomuco miehei lipase is an industrial enzyme with application in food, detergent and pharmaceutical industries. It is also used in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis. Rhizomuco miehei lipase may have potential in producing biodiesel from waste cooking oil.

Preparation Note

purified 1,3-specific lipase from Rhizomucor miehei produced by submerged fermentation of a genetically modified Rhizomucor miehei microorganism

Legal Information

A product of Novozyme corp.
Palatase is a registered trademark of Novozymes Corp.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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