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L4386

Sigma-Aldrich

L-15 Medium (Leibovitz)

With L-glutamine, powder, suitable for cell culture

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Synonym(s):
Leibovitz L15
MDL number:
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

shipped in

ambient

storage temp.

2-8°C

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This Item
L1518M7528L5520
Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M7528

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

components

phenol red: 0.011 g/L
L-glutamine: 0.3 g/L
sodium pyruvate: 0.55 g/L

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

L-15 Medium (Leibovitz) is formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate supplements. It supports established cell lines, such as human larynx epidermoid cells (HEp-2) and Rhesus monkey kidney epithelial cells (LLC-MK2), as well as primary explants of embryonic and adult human tissue. L-15 medium can also be used for culturing viruses.

Application

L-15 Medium (Leibovitz) has been used:
  • to study the toxicity of silver nanoparticles (AgNPs) with primary cultures of Eisenia fetida coelomocytes
  • to culture crab hepatopancreas and ovary tissues
  • as a component of the dissociation medium for infusion of the pancreas of mice

Quantity

Formulated to contain 13.8 grams of powder per liter of medium.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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L-15 Media Formulation

L-15 Media Formulation

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