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L5142

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

biotin conjugate, lyophilized powder

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Synonym(s):
WGA, Wheat germ agglutinin
eCl@ss:
34058011
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

form

lyophilized powder

composition

Protein, ≥80% E1%/280

extent of labeling

2-4 mol biotin per mol protein

storage temp.

2-8°C

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This Item
L0636L1882L1394
form

lyophilized powder

form

lyophilized powder

form

saline suspension

form

saline suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥80% E1%/280

composition

-

composition

-

composition

-

extent of labeling

2-4 mol biotin per mol protein

extent of labeling

-

extent of labeling

5-10 mg per mL

extent of labeling

~6 mg per mL

General description

Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.

Application

Lectin from Triticum vulgaris (wheat) has been used:
  • in lectin binding assay of samples from intrauterine stages of filarial parasites
  • in the histological studies of mice myocytes
  • for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.

Legal Information

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
R E Morris et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(5), 711-721 (1992-05-01)
We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)

Articles

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