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L5142

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

biotin conjugate, lyophilized powder

Synonym(s):

WGA, Wheat germ agglutinin

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$80.00
5 MG
$265.00

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1 MG
$80.00
5 MG
$265.00

About This Item

UNSPSC Code:
12352202
eCl@ss:
34058011
NACRES:
NA.32

$80.00

Check Cart for Availability

conjugate

biotin conjugate

Quality Level

200

form

lyophilized powder

composition

Protein, ≥80% E1%/280

extent of labeling

2-4 mol biotin per mol protein

storage temp.

2-8°C

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Lectin from Triticum vulgaris (wheat) biotin conjugate, lyophilized powder

L5142

Lectin from Triticum vulgaris (wheat)

Lectin from Triticum vulgaris (wheat) suitable for cell culture, BioReagent, lyophilized powder

L0636

Lectin from Triticum vulgaris (wheat)

Lectin from Triticum vulgaris (wheat) peroxidase conjugate, lyophilized powder

L3892

Lectin from Triticum vulgaris (wheat)

Lectin from Triticum vulgaris (wheat) lyophilized powder

L9640

Lectin from Triticum vulgaris (wheat)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

conjugate

biotin conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

-

composition

Protein, ≥80% E1%/280

composition

-

composition

Protein, ~90% modified Warburg-Christian

composition

Protein, 100% biuret

extent of labeling

2-4 mol biotin per mol protein

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins.[1] WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.[2]

Application

Lectin from Triticum vulgaris (wheat) has been used:
  • in lectin binding assay of samples from intrauterine stages of filarial parasites[3]
  • in the histological studies of mice myocytes[4]
  • for the generation bead-biotin-WGA complex with Dynabeads® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples[5]

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.

Legal Information

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000436131
0000436131
0000427379
0000427379
0000381540

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