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MilliporeSigma

L5293

Lipopolysaccharides from Escherichia coli O111:B4

Ready Made solution, 1 mg/mL

Synonym(s):

LPS

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2 ML

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About This Item

CAS Number:
93572-42-0
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00164401

Key Documents

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biological source

Escherichia coli (O111:B4)

form

aqueous solution

concentration

1 mg/mL

color

colorless to faint yellow

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Show Differences

1 of 4

This Item
L5668L5543L4130
Lipopolysaccharides from Escherichia coli O111:B4 Ready Made solution, 1 mg/mL

Sigma-Aldrich

L5293

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O127:B8 Ready Made solution, 1 mg/mL

Sigma-Aldrich

L5668

Lipopolysaccharides from Escherichia coli O127:B8

Lipopolysaccharides from Escherichia coli O26:B6 Ready Made solution, 1 mg/mL, 0.2 μm filtered

Sigma-Aldrich

L5543

Lipopolysaccharides from Escherichia coli O26:B6

Lipopolysaccharides from Escherichia coli O111:B4 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4130

Lipopolysaccharides from Escherichia coli O111:B4

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O127:B8)

biological source

Escherichia coli (O26:B6)

biological source

Escherichia coli (O111:B4)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

form

aqueous solution

form

buffered aqueous solution

form

aqueous solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

colorless to faint yellow

color

colorless to faint yellow

color

colorless to faint yellow

color

white to light yellow

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli O111:B4 has been used:
  • to stimulate an inflammatory response in porcine liver tissues in-vivo[1] and piglet ileum tissues ex vivo[2]
  • to stimulate Ex vivo natural killer(NK) cells[3] and hepatic B cells[4]
  • to induce arthritis in a standard rabbit model[5]

Biochem/physiol Actions

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.[6]

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

comparable product

Product No.
Description
Pricing

L4391

Lipopolysaccharides from Escherichia coli O111:B4, γ-irradiated, BioXtra, suitable for cell culture

L3012

Lipopolysaccharides from Escherichia coli O111:B4, purified by gel-filtration chromatography

L4130

Lipopolysaccharides from Escherichia coli O111:B4, purified by trichloroacetic acid extraction

L3024

Lipopolysaccharides from Escherichia coli O111:B4, purified by ion-exchange chromatography, TLR ligand tested

L2630

Lipopolysaccharides from Escherichia coli O111:B4, purified by phenol extraction

L3023

Lipopolysaccharides from Escherichia coli O111:B4, Detoxified

F3665

Lipopolysaccharides from Escherichia coli O111:B4, FITC conjugate

L5293

Lipopolysaccharides from Escherichia coli O111:B4, Ready Made solution, 1 mg/mL

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000477766
0000477766
0000440352
0000440352
0000408042

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Effect of maternal seaweed extract supplementation on suckling piglet growth, humoral immunity, selected microflora, and immune response after an ex vivo lipopolysaccharide challenge
Leonard SG, et al.
Journal of Animal Science, 90(2), 505-514 (2012)
Effects of dietary beta-glucans supplementation on cytokine expression in porcine liver
Ryan M, et al.
Journal of Animal Science, 90(suppl-4), 40-42 (2012)
6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Methods in Microbiology, 18(5), 157-207 (1985)
Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool
Tiwari N, et al.
Journal of Leukocyte Biology, 15(5), 056001-056001 (2010)
Ryan J Lu et al.
Nature aging, 1(8), 715-733 (2021-09-14)
Neutrophils are the most abundant human white blood cell and constitute a first line of defense in the innate immune response. Neutrophils are short-lived cells, and thus the impact of organismal aging on neutrophil biology, especially as a function of
View All Related Papers

Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Related Content

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is recommended for the storage of longer duration once opened? Is it better to keep at 2°C-8°C for 1-2 years or to freeze it (-80°C or -20°C) and thaw it gently when needed?

    1 answer

    1. This product is stable at 2-8°C at least until the assigned retest date, provided that no contaminants have been introduced. The recommended retest date is lot specific and reported in the product Certificate of Analysis. The stability of the product in dilute form or beyond the assigned retest date has not been determined. However, LPS products prepared from the powder form may be stored in 1 mg/mL aliquots for up to 2 years at -20°C. The storage of solutions at -80°C has not been determined. Please see the link below to review additional information regarding the stability and preparation of Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Helpful?

  4. What solvent is the product L5293-2ML dissolved in?

    1 answer

    1. The solvent information for this product is proprietary, but it is acknowledged to contain water, while the remaining components remain undisclosed.

      Helpful?

  5. Bonjour, Comment ce LPS a t'il été extrait / Purifié ?

    1 answer

    1. This product is a sterile filtered solution. The extraction method for this material is considered proprietary, however it is chromatographically purified.

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