L5505

Lectin from Ulex europaeus (gorse, furze)

lyophilized powder

• Synonym(s): Ulex europaeus agglutinin, UEA-I
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• potency: <8 μg/mL agglutination activity
• composition: Protein, ≥80% biuret
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Linkage

Conjugates are prepared from affinity purified lectin.

Preparation Note

Purified by affinity chromatography using the method of Pereira.[1]

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)