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L6135

Sigma-Aldrich

Lectin from Arachis hypogaea (peanut)

biotin conjugate, lyophilized powder

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Synonym(s):
PNA, Peanut agglutinin
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

form

lyophilized powder

mol wt

120 kDa

composition

Protein, ≥80% Lowry

extent of labeling

~3 mol biotin per mol protein

technique(s)

blood typing: suitable
fractionation: suitable

solubility

water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless

storage temp.

2-8°C

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This Item
L7759L7381L4144
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

120 kDa

mol wt

-

mol wt

-

mol wt

-

technique(s)

blood typing: suitable, fractionation: suitable

technique(s)

western blot: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

solubility

water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless

solubility

-

solubility

-

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Peanut lectin (PNA) is a lectin or an agglutinin from the legume, Arachis hypogaea, found in peripheral venous blood in humans after ingestion of peanuts. PNA lectin is specific to the galactosyl (β-1,3) N-acetylgalactosamine.

Application

Lectin from Arachis hypogaea (peanut) has been used:
  • in enzyme-linked micro plate assay to detect β-D-galactose-N-acetylglucosamine
  • in Immunohistochemical double staining
  • Immunohistochemical double staining for the detection of germinal centers
  • in enzyme-linked microplate assayto detect β-D-galactose-N-acetylglucosamine

Biochem/physiol Actions

Lectin from Arachis hypogaea (peanut) functions as an antigenic determinant for Thomsen-Friedenreich antigen (TF) and as mitogen for colorectal cancer stimulation cell division in normal colonic epithelium. It is therefore used as tumor marker.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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