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L6261

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype minnesota

purified by phenol extraction

Synonym(s):
LPS
MDL number:
NACRES:
NA.83

form

lyophilized powder

Quality Level

purified by

phenol extraction

impurities

<3% Protein (Lowry)

storage temp.

2-8°C

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General description

Lipopolysaccharide (LPS) is the pyrogenic element arising from enterobacteria such as Escherichia coli and Salmonella enterica. It is an amphiphilic component containing polysaccharides and lipoidic material. LPS is present in the outer membrane of the cell wall of almost all Gram-negative bacteria.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Application

Lipopolysaccharides from Salmonella enterica serotype minnesota has been used:
  • as a toll-like receptors (TLR ) agonist to induce tumor necrosis factor (TNF) α production in cereblon and Rabex-5 deficient mice in vivo,
  • to study its effect on prostaglandin-E Synthase (PTGES) protein expression in monocyte-derived macrophages
  • as a positive control for the maturation of monocyte-derived dendritic cells (MoDCs) to assess secretion of interleukin-10 and interferon (IFN)-γ
  • as a supplement in Roswell park memorial institute (RPMI)media for culturing splenic B cells
  • as a toll-like receptor 4 ligand to study its effect on the expression of the proallergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL33) in H292 cells (pulmonary mucoepidermoid carcinoma cells)

Biochem/physiol Actions

Lipopolysaccharide (LPS) forms a permeability barrier around the cell surface to restrict the entry of toxic molecules such as antibiotics and bile salts. Owing to their external location, this endotoxin also plays a vital role in host-bacterium interactions by regulating host immune response. LPS is a microbe-associated molecular pattern (MAMP) that serves as a potent agonist for toll-like receptor 4 (TLR4)-MD2-CD14 receptor complex.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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