L6533

Lectin from Dolichos biflorus (horse gram)

Name: Lectin from <I>Dolichos biflorus</I> (horse gram)
Brand: SIGMA

biotin conjugate, buffered aqueous solution

Synonym(s):

Dolichos biflorus agglutinin, DBA

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Key Documents

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conjugate

biotin conjugate

form

buffered aqueous solution

composition

Protein, ≥2.5 mg/mL biuret

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

Quality Level

200

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This Item	L2785	L6512	L2895
Sigma-Aldrich L6533 Lectin from Dolichos biflorus (horse gram)	Sigma-Aldrich L2785 Lectin from Dolichos biflorus (horse gram)	Sigma-Aldrich L6512 Lectin from Helix pomatia	Sigma-Aldrich L2895 Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
form buffered aqueous solution	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
conjugate biotin conjugate	conjugate -	conjugate biotin conjugate	conjugate FITC conjugate
composition Protein, ≥2.5 mg/mL biuret	composition -	composition -	composition Protein, ≥70% Lowry (balance sodium citrate)
extent of labeling ≥3 mol biotin per mol lectin	extent of labeling -	extent of labeling 2-4 mol biotin per mol protein	extent of labeling ≥1 mol FITC per mol lectin

Application

Lectin from Dolichos biflorus (horse gram) has been used:

to characterize mouse uterine natural killer (uNK) cells[1]
in double staining with lectins to localize nuclear factor-κB (NF-κB) activation[2]
as a species-specific gonocyte marker for the determination of donor cell colonization[3]
to label undifferentiated spermatogonia/cat testicular tissue[4]

Biochem/physiol Actions

DBA has anti-A₁ human blood group specificity, useful for distinguishing between A₁ and A₂ blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation.[5] It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.[4]

General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.[6]

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin

Escada-Rebelo S, et al.

Andrology, 4(6), 1159-1168 (2016)

Analysis of uterine natural killer cells in mice.

B Anne Croy et al.

Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)

The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment

Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells

Nakano H, et al.

Reproduction (Cambridge, England), 124(4), 581-592 (2002)

Dose-Dependent Cardioprotection of Moderate (32°C) Versus Mild (35°C) Therapeutic Hypothermia in Porcine Acute Myocardial Infarction.

Rajesh Dash et al.

JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)

The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.

Effect of horse gram lectin (Dolichos biflorus agglutinin) on degranulation of mast cells and basophils of atopic subjects: Identification as an allergen

Pramod SN, et al.

International Immunopharmacology, 6(11), 1714-1722 (2006)

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