MilliporeSigma
All Photos(1)

Documents

L6533

Sigma-Aldrich

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Dolichos biflorus agglutinin, DBA
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

form

buffered aqueous solution

composition

Protein, ≥2.5 mg/mL biuret

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
L1882L9017L5142
storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

conjugate

biotin conjugate

conjugate

agarose conjugate

conjugate

-

conjugate

biotin conjugate

composition

Protein, ≥2.5 mg/mL biuret

composition

-

composition

-

composition

Protein, ≥80% E1%/280

extent of labeling

≥3 mol biotin per mol lectin

extent of labeling

5-10 mg per mL

extent of labeling

-

extent of labeling

2-4 mol biotin per mol protein

General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.

Application

Lectin from Dolichos biflorus (horse gram) has been used:
  • to characterize mouse uterine natural killer (uNK) cells
  • in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
  • as a species-specific gonocyte marker for the determination of donor cell colonization
  • to label undifferentiated spermatogonia/cat testicular tissue

Biochem/physiol Actions

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Tubular NF-kB and AP-1 activation in human proteinuric renal disease
Mezzano SA, et al.
Kidney International, 60(4), 1366-1377 (2001)
Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures
Zhang Z, et al.
Journal of Andrology, 29(4), 418-430 (2008)
Effect of horse gram lectin (Dolichos biflorus agglutinin) on degranulation of mast cells and basophils of atopic subjects: Identification as an allergen
Pramod SN, et al.
International Immunopharmacology, 6(11), 1714-1722 (2006)
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
Analysis of uterine natural killer cells in mice
Croy BA, et al.
Natural Killer Cell Protocols, 465-503 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service