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L6544
LuminoCt® SYBR® Green qPCR ReadyMix™
For fast SYBR Green quantitative PCR
Recommended Products
Quality Level
form
liquid
usage
sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions
feature
dNTPs included
hotstart
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
application(s)
agriculture
compatibility
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
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Related Categories
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This Item | D7440 | KCQS02 | KCQS01 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| usage sufficient for 100 reactions | usage sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions |
| feature dNTPs included | feature Multiplex PCR, dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| color colorless | color colorless | color colorless | color colorless |
| input purified DNA | input purified DNA | input purified DNA | input purified DNA |
General description
- Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes
Application
Features and Benefits
- Ideal for performing high-throughput, quantitative PCR applications
- The master mix allows consistency from one reaction to the next
- Reduced preparation time and reduced risk of contamination from multiple pipetting steps
- The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
- This master mix is compatible with commercial primer sets, including TaqMan® assays
- Assay results in as little as 25 minutes
- Unsurpassed accuracy, precision, and sensitivity
- Virtually eliminates the need to optimize assay parameters
Packaging
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
- At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
- Sigma recommends designing primers and probes with Beacon Designer software
- To order primers and probes from Sigma, visit: www.sigma.com/oligos
Legal Information
Kit Components Only
- 2 X
Kit Components Also Available Separately
- R4526100X ROX internal reference dyeSDS
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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Articles
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Protocols
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
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