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L6910

Sigma-Aldrich

In Vivo Liposome Transfection Reagent

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storage temp.

2-8°C

Biochem/physiol Actions

When DNA is mixed with the DOTAP:cholesterol (1:1 molar ratio) extruded liposomes, it becomes condensed in the liposome interior. The liposomes are stable at high concentration and with a high concentration of DNA, allowing large quantities of DNA to be delivered in vivo. Transfection efficiency is correspondingly high. Transfection of cells in animals provides a tool to study gene expression in vivo, to alter gene expression, and to express foreign genes in the living host.

Other Notes

Liposomes extruded from 20 mM DOTAP, 20 mM cholesterol in 5% glucose.

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N S Templeton et al.
Nature biotechnology, 15(7), 647-652 (1997-07-01)
To increase cationic liposome-mediated intravenous DNA delivery extruded DOTAP:cholesterol liposomes were used to form complexes with DNA, resulting in enhanced expression of the chloramphenicol acetyltransferase gene in most tissues examined. The DNA:liposome ratio, and mild sonication, heating, and extrusion steps

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