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L7533

Sigma-Aldrich

LB Broth with agar (Lennox)

EZMix® powder microbial growth medium

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NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L2897L7658L3147
vibrant-m

L7533

LB Broth with agar (Lennox)

vibrant-m

L2897

LB Broth with agar (Lennox)

vibrant-m

L7658

LB Broth (Lennox)

vibrant-m

L3147

LB Broth with agar (Miller)

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

composition

Agar, 15 g/L , Tryptone, 10 g/L , NaCl, 5 g/L , Yeast Extract, 5 g/L

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

-

composition

Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox EZMix® powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Preparation Note

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Reconstitution

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany
Zeocin is a registered trademark of Cayla Sarl

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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