L7543

Sigma-Aldrich

Anti-LC3B antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-MAP1A/MAP1B LC3 B, Anti-Microtubule-associated proteins 1A/B light chain 3B precursor, LC3B Antibody - Anti-LC3B antibody produced in rabbit, Anti-Autophagy-related protein LC3 B, Lc3 Antibody Sigma, Anti-MAP1 light chain 3-like protein 2, Anti-Autophagy-related ubiquitin-like modifier LC3 B, Anti-MAP1A/1B light chain 3 B, Lc3B Antibody
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

rat, human, mouse

enhanced validation

functional assay
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

application(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues.
The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Specificity

By immunoblotting, the antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively).

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse.

Application

Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) expression level correlates with the extent of autophagosome formation. LC3B can be used as an autophagosomal marker.
MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
Gomez-Sanchez R
Data in Brief, 7(7), 641-647 (2016)
Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus
Zheng HY, et al.
Cancer Biology & Medicine, 9(2), 105-105 (2012)
Yehui Duan et al.
Food & function, 10(8), 5152-5165 (2019-08-03)
The aim of this study was to investigate the effects of dietary β-hydroxy-β-methylbutyrate (HMB) on lipopolysaccharide (LPS)-induced muscle atrophy and to investigate the mechanisms involved. Sixty pigs (21 ± 2 days old, 5.86 ± 0.18 kg body weight) were used...
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Kabeya Y, et al.
The Embo Journal, 19(21), 5720-5728 (2000)
Robert W Gilkerson et al.
Human molecular genetics, 21(5), 978-990 (2011-11-15)
Autophagy has emerged as a key cellular process for organellar quality control, yet this pathway apparently fails to eliminate mitochondria containing pathogenic mutations in mitochondrial DNA (mtDNA) in patients with a variety of human diseases. In order to explore how...
Articles
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.