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L7658

Sigma-Aldrich

LB Broth (Lennox)

EZMix® powder microbial growth medium

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Synonym(s):
Lennox broth
NACRES:
NA.85

grade

for molecular biology

Quality Level

description

quick-dissolve, dust-free formulation

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.5% solution)

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L3022L7275L2897
LB Broth (Lennox) EZMix® powder microbial growth medium

Sigma-Aldrich

L7658

LB Broth (Lennox)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

LB Broth (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7275

LB Broth (Lennox)

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

tablet

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.5% solution)

pH

6.8-7.2(2% solution)

pH

-

pH

-

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB EZMix® powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Components

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)

Preparation Note

1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.

Reconstitution

Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

EZMix is a registered trademark of Merck KGaA, Darmstadt, Germany
Zeocin is a registered trademark of Cayla Sarl

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Katherine A Kantardjieff et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 5), 895-902 (2004-04-23)
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Protocols

General protocols for growth of competent cells in microbial medium.

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