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L7759
Lectin from Arachis hypogaea (peanut)
peroxidase conjugate, lyophilized powder
Synonym(s):
PNA, Peanut agglutinin
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250 μG
$136.42
1 MG
$483.00
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250 μG
$136.42
1 MG
$483.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
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biological source
Arachis hypogaea (peanut)
Quality Level
conjugate
peroxidase conjugate
form
lyophilized powder
peroxidase activity
≥20 units/mg protein
technique(s)
western blot: suitable
storage temp.
−20°C
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Related Categories
1 of 4
This Item | L7381 | L8146 | L4144 |
|---|---|---|---|
| biological source Arachis hypogaea (peanut) | biological source - | biological source Ulex europaeus | biological source - |
| technique(s) western blot: suitable | technique(s) - | technique(s) western blot: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate peroxidase conjugate | conjugate FITC conjugate | conjugate peroxidase conjugate | conjugate - |
Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Unit Definition
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical form
Contains sodium citrate
Preparation Note
Prepared from peroxidase (P8375) using a modification of a published method[1], which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
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Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
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