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L8005

Sigma-Aldrich

β-Lactoglobulin B from bovine milk

≥90% (PAGE)

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Synonym(s):
β-LG, Bos d 5, beta-LG
CAS Number:
MDL number:
NACRES:
NA.61

biological source

bovine milk

Assay

≥90% (PAGE)

form

powder

mol wt

18,276 Da by calculation

technique(s)

HPLC: suitable
electrophoresis: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... LGB(280838)

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assay

≥90% (PAGE)

assay

≥90% (PAGE)

assay

≥90% (PAGE)

assay

≥85% (PAGE)

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

18,276 Da by calculation

mol wt

-

mol wt

-

mol wt

-

technique(s)

HPLC: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

ELISA: suitable

technique(s)

titration: suitable

UniProt accession no.

P02754

UniProt accession no.

P02754

UniProt accession no.

P02754

UniProt accession no.

P02754

General description

β-Lactoglobulin B (β-LG B), a isoform of β-Lactoglobulin, is a small protein of 162 amino acids with a molecular mass of 18.2 kDa and optimum pH of 5.2. It is present in high level in casein and is relatively low in raw bovine milk. β-LG has eight-stranded β-barrel (strands A-H) succeeded by a three-turn α-helix and a final β-strand (strand I), that forms part of the dimerization interface.
Milk from dairy cows contains the protein β-lactoglobulin (BLG). It naturally occurs in a number of genetic variants, and the most prevalent bovine variants are known as BLG A and BLG B.

Application

β-Lactoglobulin B from bovine milk has been used as:
  • a protein standard in SDS-polyacrylamide electrophoresis for quantification of milk protein fractions
  • in the calibration of reversed phase- high-performance liquid chromatography (HPLC) for caseins quantification
  • for immobilization on the biosensor surface and a calibration standards in biosensor assay

β-Lactoglobulin was used in the identification of the genetic variants of κ-casein in milk by isoelectric focusing electrophoresis.

Biochem/physiol Actions

β-Lactoglobulin B (β-LG B) show less inhibitory effect on the Staphylaococcus sp compared to β-LG A.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The interaction between whey carrier protein β-lactoglobulin B and (-)-epicatechin, a major dietary flavonoid with a wide range of health-promoting biological activities, was investigated by Fourier transform infrared spectroscopy in physiological conditions. Amide I spectra of epicatechin - β-lactoglobulin complexes

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