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L8146
Lectin from Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
Lectin from Ulex europaeus (gorse, furze)
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Synonym(s):
Ulex europaeus agglutinin, UEA-I
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.5 mg | Available to ship TODAYfromMILWAUKEE | $362.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
70.00-90.00% (Lowry)
Biological source:
Ulex europaeus
$362.00
Available to ship TODAYDetails
biological source
Ulex europaeus
Quality Level
conjugate
peroxidase conjugate
assay
70.00-90.00% (Lowry)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
composition
Protein, ~80% Lowry
technique(s)
western blot: suitable
storage temp.
2-8°C
Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate buffer salts
Preparation Note
Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.[1]
Repurified by affinity chromatography after conjugation.
Repurified by affinity chromatography after conjugation.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Other Notes
Conjugates are prepared from affinity purified lectin.
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
1 of 1
This Item | |||
|---|---|---|---|
| assay 70.00-90.00% (Lowry) | assay - | assay - | assay - |
| biological source Ulex europaeus | biological source - | biological source - | biological source Bandieraea simplicifolia |
| technique(s) western blot: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)
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The IκB kinase (IKK)-NF-κB signaling pathway plays a multifaceted role in inflammatory bowel disease (IBD): on the one hand, it protects from apoptosis; on the other, it activates transcription of numerous inflammatory cytokines and chemokines. Although several murine models of
K Nozaki et al.
Journal of neurology, neurosurgery, and psychiatry, 80(8), 904-908 (2009-02-26)
To study the clinical and pathological correlations of neuromuscular patients with a high aldolase and normal creatine kinase (CK) in serum at presentation or during a symptomatic exacerbation. Records and muscle biopsies were retrospectively reviewed in a consecutive series of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L8146-1MG | 04061826738948 |
| L8146-.5MG | 04061833421161 |