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L8262

Lectin from Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

Synonym(s):

Ulex europaeus agglutinin, UEA-I

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2 mg
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$339.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder

$339.00

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conjugate

biotin conjugate

Quality Level

200

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~85% E1%/280

extent of labeling

~3 mol biotin per mol protein

storage temp.

2-8°C

Application

Lectin from Ulex europaeus (gorse, furze) has been used:
  • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution [1]
  • in double staining for endothelial cells for quantitation of blood vessels[2]
  • in histochemical staining of bronchial epithelial cells[3]

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.

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Sigma-Aldrich

L9640

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form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

-

potency

<50 μg/mL agglutination activity

potency

<5 μg/mL agglutination activity

potency

<8 μg/mL agglutination activity

potency

<20 μg/mL agglutination activity

extent of labeling

~3 mol biotin per mol protein

extent of labeling

2-5 mol FITC per mol protein

extent of labeling

-

extent of labeling

-

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000544285
0000544285
0000453637
0000453637
0000452384

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Articles

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
The aggregation of lymphoid cells in the bronchial mucosa has been named the bronchus-associated lymphoid tissue (BALT) and investigated in comparison with the gut-associated lymphoid tissue (GALT). To elucidate precisely the structure and function of the BALT, the present study
Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
Maria Hernandez et al.
Biomedicines, 9(11) (2021-11-28)
The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal
View All Related Papers

Global Trade Item Number

SKUGTIN
L8262-2MG04061833965931

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