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L8271

Sigma-Aldrich

Monoclonal Anti-Laminin antibody produced in mouse

clone LAM-89, ascites fluid

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MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LAM-89, monoclonal

contains

15 mM sodium azide

species reactivity

feline, human, pig

should not react with

rabbit, lizard, sheep, carp, canine, chicken, rat, guinea pig, goat, frog, snake

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using enzyme-treated human tissue sections
indirect ELISA: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
SAB4200719L9393WH0003911M1
species reactivity

feline, human, pig

species reactivity

feline, human, porcine

species reactivity

animal, human

species reactivity

human

Gene Information

human ... LAMA1(284217), LAMA2(3908), LAMB1(3912), LAMB2(3913)

Gene Information

cat ... Lama1(101083299)
human ... LAMA1(284217)
pig ... Lama1(100625589)

Gene Information

human ... LAMA1(284217)
mouse ... Lama1(16772)

Gene Information

human ... LAMA5(3911)

clone

LAM-89, monoclonal

clone

LAM-89, monoclonal

clone

polyclonal

clone

2F7, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

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General description

Laminin, a 900kD (approx.) complex extracellular glycoprotein, is the most abundant structural and biologically active component present in the basement membranes. It is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement. Monoclonal Anti-laminin antibody can be used in immunohistochemistry to classify various disease processes involving basement membranes, to mark blood vessel walls of different species and to determine the origin and classification of human tumours. It can also be used in dot blot assays. Mouse anti- laminin antibody reacts specifically with purified human laminin but not with collagen IV, fibronectin, vitronectin or chondroitin sulfate types A, B, and C.
Monoclonal Anti-Laminin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle.

Immunogen

human laminin.

Application

Monoclonal Anti-Laminin antibody produced in mouse has been used in:
  • immunohistochemistry to stain matrix proteins
  • double immunofluorescence staining
  • indirect immunofluorescence
  • immunogold labelling for electron microscopy

Monoclonal Anti-laminin antibody can be used in indirect immunostaining to determine the distribution of laminin. It may also be used for ELISA, Immunofluorescence and western blotting.

Biochem/physiol Actions

Laminin is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement because it appears to be an important cell substrate adhesion protein. It plays an important role in many aspects of the cell biology. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Enhanced expression of matrilysin, collagenase, and stromelysin-1 in gastrointestinal ulcers.
Saarialho-Kere UK, et al.
The American Journal of Pathology, 148(2), 519-519 (1996)
Bryon R McKay et al.
The Journal of physiology, 588(Pt 17), 3307-3320 (2010-07-14)
In humans, muscle satellite cell (SC) enumeration is an important measurement used to determine the myogenic response to various stimuli. To date, the standard practice for enumeration is immunohistochemistry (IHC) using antibodies against common SC markers (Pax7, NCAM). Flow cytometry
Sandra Liekens et al.
Molecular cancer therapeutics, 11(4), 817-829 (2012-02-04)
5'-O-Tritylinosine (KIN59) is an allosteric inhibitor of the angiogenic enzyme thymidine phosphorylase. Previous observations showed the capacity of KIN59 to abrogate thymidine phosphorylase-induced as well as developmental angiogenesis in the chicken chorioallantoic membrane (CAM) assay. Here, we show that KIN59
Laminin biosynthesis in the extracellular matrix-producing cell line PFHR9 studied with monoclonal and polyclonal antibodies
Lissitzky JC, et al.
The Biochemical Journal, 250(3), 843-852 (1988)
Activation of hepatocyte growth factor receptor, c-met, in renal tubules is required for renoprotection after acute kidney injury
Zhou D, et al.
Kidney International, 84(3), 509-520 (2013)

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