L9031

Lipase from Mucor miehei

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

• Synonym(s): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS Number: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• EC Number: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ≥4,000 units/mg solid (using olive oil)

Properties

• form: lyophilized powder
• Quality Level: 100
• specific activity: ≥4,000 units/mg solid (using olive oil)
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipase is produced by several animals, microbes, and plants. Lipase from Mucor sp. is a fungal lipase. It is a member of the serine hydrolase family.[1]

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters. [2]

Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. [3] It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor. [4]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Microbial lipase has a wide industrial application due to its stability, broad substrate specificity, and selectivity. It may be used in the synthesis of biosurfactants and finds application in organic chemical processing, dairy industry, oleochemical industry, detergent formulations, paper, cosmetics, nutrition, and the pharmaceutical industry.[5]

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)