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L9150

N-Lauroylsarcosine sodium salt

detergent for use in cell lysis

Synonym(s):

Sodium lauroyl sarcosinate, N-Dodecanoyl-N-methylglycine sodium salt, Sarkosyl NL

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50 g

Available to ship TODAYfromMILWAUKEE

$48.40
100 g

Available to ship TODAYfromMILWAUKEE

$82.90
250 g

Available to ship TODAYfromMILWAUKEE

$171.00

About This Item

Linear Formula:
CH3(CH2)10CON(CH3)CH2COONa
CAS Number:
Molecular Weight:
293.38
UNSPSC Code:
12161902
NACRES:
NA.52
PubChem Substance ID:
EC Number:
205-281-5
Beilstein/REAXYS Number:
5322974
MDL number:

$48.40


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grade

Molecular Biology

Quality Level

description

anionic

product line

BioReagent

assay

≥94%

form

powder

mol wt

micellar avg mol wt 600

aggregation number

2

technique(s)

protein purification: suitable, protein quantification: suitable

CMC

14.6 mM (20-25°C)

solubility

H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

[Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O

InChI

1S/C15H29NO3.Na/c1-3-4-5-6-7-8-9-10-11-12-14(17)16(2)13-15(18)19;/h3-13H2,1-2H3,(H,18,19);/q;+1/p-1

InChI key

KSAVQLQVUXSOCR-UHFFFAOYSA-M

General description

Sodium lauroyl sarcosinate, the sodium salt of an acyl derivative of sarcosine—a natural amino acid occurring in muscles and body tissues—stands out as a valuable component in molecular biology, biochemical, and cell biology research. Derived from a natural fatty acid and the amino acid derivative sarcosine, N-Lauroylsarcosine is a biodegradable anionic surfactant usually synthesized through acylation and neutralization reactions involving lauric acid and sodium sarcosine.

This amino acid-type anionic surfactant, also recognized as a fatty acid amide and detergent, exhibits protein denaturant potency and has been integrated into lysing solutions, such as the cornet assay. Its application extends to lysing protoplasts for chromosomal DNA isolation from Streptococcus mutans. Notably, Lauroylsarcosine Sodium Salt, characterized by its ionic detergent properties, is commonly employed for purifying membrane proteins. Additionally, Sodium Lauroyl Sarcosine also plays a crucial role in concentrated salt solutions, facilitating cell lysis in RNA purification protocols and membrane solubilization. Whether it′s breaking down cell structures or facilitating membrane protein purification, this mild surfactant proves valuable in various applications within the realms of biology and biochemistry.

Application

Suitable for
  • solubilization and separation of membrane proteins
  • lysis of cells during the isolation of RNA
  • inhibition of hexokinase

Features and Benefits

  • Suitable for Molecular Biology, Biochemical and Cell Biology research
  • Can be used for Protein Purification and Quantification
  • High purity product for research applications

Other Notes

Anionic surfactant.
For additional information on our range of Biochemicals, please complete this form.

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This Item
L5777428010L5125
technique(s)

protein purification: suitable, protein quantification: suitable

technique(s)

-

technique(s)

-

technique(s)

oligo synthesis: suitable

form

powder

form

powder

form

solid

form

powder

assay

≥94%

assay

≥97% (TLC)

assay

≥99% (HPLC)

assay

≥94%

grade

Molecular Biology

grade

-

grade

-

grade

-

aggregation number

2

aggregation number

2

aggregation number

-

aggregation number

2

storage temp.

room temp

storage temp.

-

storage temp.

15-25°C

storage temp.

-


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pictograms

Skull and crossbonesCorrosion

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Eye Dam. 1 - Skin Irrit. 2

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

512.6 °F - closed cup

flash_point_c

267 °C - closed cup

ppe

Eyeshields, Gloves, type N95 (US)



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M Swenshon et al.
Journal of clinical microbiology, 36(7), 1902-1906 (1998-07-03)
The present study was designed to identify and comparatively investigate 35 beta-hemolytic streptococci isolated from stranded harbor porpoises or from animals caught in fishing nets of the North and Baltic seas. According to biochemical and serological data and to lectin
A R Günthert et al.
The Journal of cell biology, 134(4), 1089-1096 (1996-08-01)
Ligation of CD95 (APO-1/Fas) cell surface receptors induces death in apoptosis-sensitive cells. Induction of apoptosis in adherent gamma interferon-stimulated HT-29 and COLO 205 colon carcinoma cells by cross-linking CD95 with anti-APO-1 monoclonal antibody resulted in detachment of the cells from
Rhonda I Hobb et al.
Microbiology (Reading, England), 155(Pt 3), 979-988 (2009-02-28)
Although infection with Campylobacter jejuni is one of the leading causes of gastroenteritis worldwide, relatively little is known about the factors that are required to elicit a protective immune response. The need for a vaccine against this pathogen is well



Global Trade Item Number

SKUGTIN
L9150-100G04061835546565
L9150-250G04061835546572
L9150-50G04061835546589

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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