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L9294

Sigma-Aldrich

Anti-LRP6 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-CD, Anti-Coronary artery disease, autosomal dominant 2, Anti-Low-density lipoprotein receptor-related protein 6, Anti-ADCAD2
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~180 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

application(s)

immunoprecipitation (IP): 5-10 μg using lysate of HEK-293T cells expressing human LRP6
western blot: 1-2 μg/mL using lysate of HEK-293T cell expressing human LRP6.

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LRP6(4040)
mouse ... Lrp6(16974)
rat ... Lrp6(312781)

General description

Low-density lipoprotein receptor-related protein 6 (LRP6) belongs to the low-density lipoprotein receptor (LDLR) family and is expressed majorly in the nervous system. It is closely related to LRP5. LRP6 gene is mapped to human chromosome 12p13.2.

Specificity

Anti-LRP6 specifically recognizes human LRP6.

Application

Anti-LRP6 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Low-density lipoprotein receptor related protein 6 (LRP6) is part of the wingless (Wnt)-frizzled-LRP6 complex, that recruits axin to the plasma membrane. This results in the inhibition of beta-catenin phosphorylation and subsequent degradation and activation of T-cell factor/lymphoid enhancer-binding factor 1 (TCF/LEF-1)-dependent transcription. LRP6 is essential for proper brain development. Deletion of the LRP6 gene results in severe brain abnormalities. Mutations in LRP6 are associated with coronary heart disease and osteoporosis. LRP6 has been shown to undergo phosphorylation at multiple PPPSP motifs upon stimulation by Wnt. The phosphorylation of LRP6 by glycogen synthase kinase 3 (GSK3) and casein kinase 1 (CKI) is induced by Wnt. Regulated proteolytic processing of LRP6 has shown to release its intracellular domain (ICD). This fragment binds to GSK3 and inhibits its activity, resulting in activation of the canonical Wnt signaling pathway. A higher expression of LRP6 is detected in tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

1

Certificate of Analysis
Certificate of Origin
W Kong et al.
European review for medical and pharmacological sciences, 22(12), 3804-3811 (2018-06-28)
MiR-381-3p plays an essential role in the progression of a variety of cancers, but its expression and role in papillary thyroid carcinoma (PTC) progression have not been investigated. The aim of this study was to investigate the expression of miR-381-3p...
Arya Mani et al.
Science (New York, N.Y.), 315(5816), 1278-1282 (2007-03-03)
Coronary artery disease (CAD) is the leading cause of death worldwide and is commonly caused by a constellation of risk factors called the metabolic syndrome. We characterized a family with autosomal dominant early CAD, features of the metabolic syndrome (hyperlipidemia...
Kaihong Mi et al.
Journal of neurochemistry, 101(2), 517-529 (2007-03-01)
Low-density lipoprotein receptor-related protein 6 (LRP6) is a member of low-density lipoprotein receptor (LDLR) family which cooperates with Frizzled receptors to transduce the canonical Wnt signal. As a critical component of the canonical Wnt pathway, LRP6 is essential for appropriate...
Xin Zeng et al.
Nature, 438(7069), 873-877 (2005-12-13)
Signalling by the Wnt family of secreted lipoproteins has essential functions in development and disease. The canonical Wnt/beta-catenin pathway requires a single-span transmembrane receptor, low-density lipoprotein (LDL)-receptor-related protein 6 (LRP6), whose phosphorylation at multiple PPPSP motifs is induced upon stimulation...
Catherine M Willis et al.
PloS one, 9(8), e103966-e103966 (2014-08-05)
Expression of the glycosaminoglycan chondroitin sulfate-E (CS-E) is misregulated in many human cancers, including breast cancer. Cell-surface associated CS-E has been shown to have pro-tumorigenic functions, and pharmacological treatment with exogenous CS-E has been proposed to interfere with tumor progression...

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