MilliporeSigma
All Photos(7)

Documents

L9393

Sigma-Aldrich

Anti-Laminin antibody produced in rabbit

enhanced validation

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Laminin Antibody
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

animal, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

0.5 mg/mL

technique(s)

dot blot: 1:1,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues
microarray: suitable

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LAMA1(284217)
mouse ... Lama1(16772)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200719L8271HPA001908
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

affinity isolated antibody

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

100

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

UniProt accession no.

P25391

UniProt accession no.

P25391

UniProt accession no.

P07942, P25391

UniProt accession no.

P11047

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.

Specificity

Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.

Immunogen

Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

Application

Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.
Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.

Biochem/physiol Actions

LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Autoimmunity against laminins.
Florea F, et al.
Clinical Immunology (Orlando, Fla.), 39-52 (2016)
Yongchol Shin et al.
American journal of physiology. Cell physiology, 317(2), C314-C325 (2019-06-13)
Angiogenesis is a process through which new blood vessels are formed by sprouting and elongating from existing blood vessels. Several methods have been used to replicate angiogenesis in vitro, including culturing vascular endothelial cells on Matrigel and coculturing with endothelial
Prostatic Intraepithelial Neoplasia in Genetically Engineered Mice
Jae-Hak P, et al.
The American Journal of Pathology, 161(2), 727-735 (2002)
Zui Zhang et al.
Nature communications, 10(1), 3561-3561 (2019-08-10)
Protein corona presents a major obstacle to bench-to-bedside translation of targeted drug delivery systems, severely affecting targeting yields and directing unfavorable biodistribution. Corona-mediated targeting provides a new impetus for specific drug delivery by precisely manipulating interaction modes of functional plasma
Solving an enigma: Arterial pole development in the zebrafish heart
Adrian CG, et al.
Developmental Biology, 290(2), 265-276 (2006)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service