Documents

L9419

Sigma-Aldrich

Anti-LRP6 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-CD, Anti-Coronary artery disease, autosomal dominant 2, Anti-Low-density lipoprotein receptor-related protein 6
Pricing and availability is not currently available.

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~180 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

application(s)

immunoprecipitation (IP): 4-8 μg using lysate of HEK-293T cells expressing human LRP6
western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human LRP6

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... LRP6(4040)
mouse ... Lrp6(16974)

General description

Low-density lipoprotein receptor-related protein 6 (LRP6) gene is localized to human chromosome 12p13.2. LRP6 is expressed majorly in the nervous system and belongs to the low-density lipoprotein receptor (LDLR) family.

Specificity

Anti-LRP6 (C-terminal) specifically recognizes human LRP6.

Application

Anti-LRP6 (C-terminal) antibody produced in rabbit may be used immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Low-density lipoprotein receptor related protein 6 (LRP6) is essential for proper brain development. LRP6 has been shown to undergo phosphorylation at multiple PPPSP motifs upon stimulation by wingless (wnt). The phosphorylation of LRP6 by glycogen synthase kinase 3 (GSK3) and casein kinase 1 (CKI) is induced by Wnt. Regulated proteolytic processing of LRP6 has shown to release its intracellular domain (ICD). This further binds to GSK3 and inhibits its activity, resulting in activation of the canonical Wnt signaling pathway. The wnt-frizzled-LRP6 complex recruits axin to the plasma membrane. This leads to the inhibition of β-catenin phosphorylation, degradation and activation of T-cell factor/lymphoid enhancer-binding factor 1 (TCF/LEF-1)-dependent transcription. Deletion of the LRP6 gene results in severe brain abnormalities. Mutations in LRP6 are associated with coronary heart disease and osteoporosis. LRP6 is highly expressed in tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

1

Certificate of Analysis
Certificate of Origin
Arya Mani et al.
Science (New York, N.Y.), 315(5816), 1278-1282 (2007-03-03)
Coronary artery disease (CAD) is the leading cause of death worldwide and is commonly caused by a constellation of risk factors called the metabolic syndrome. We characterized a family with autosomal dominant early CAD, features of the metabolic syndrome (hyperlipidemia...
Kaihong Mi et al.
Journal of neurochemistry, 101(2), 517-529 (2007-03-01)
Low-density lipoprotein receptor-related protein 6 (LRP6) is a member of low-density lipoprotein receptor (LDLR) family which cooperates with Frizzled receptors to transduce the canonical Wnt signal. As a critical component of the canonical Wnt pathway, LRP6 is essential for appropriate...
W Kong et al.
European review for medical and pharmacological sciences, 22(12), 3804-3811 (2018-06-28)
MiR-381-3p plays an essential role in the progression of a variety of cancers, but its expression and role in papillary thyroid carcinoma (PTC) progression have not been investigated. The aim of this study was to investigate the expression of miR-381-3p...
Xin Zeng et al.
Nature, 438(7069), 873-877 (2005-12-13)
Signalling by the Wnt family of secreted lipoproteins has essential functions in development and disease. The canonical Wnt/beta-catenin pathway requires a single-span transmembrane receptor, low-density lipoprotein (LDL)-receptor-related protein 6 (LRP6), whose phosphorylation at multiple PPPSP motifs is induced upon stimulation...

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