Low-density lipoprotein receptor-related protein 6 (LRP6) gene is localized to human chromosome 12p13.2. LRP6 is expressed majorly in the nervous system and belongs to the low-density lipoprotein receptor (LDLR) family.
Anti-LRP6 (C-terminal) specifically recognizes human LRP6.
Anti-LRP6 (C-terminal) antibody produced in rabbit may be used immunoblotting and immunoprecipitation.
Low-density lipoprotein receptor related protein 6 (LRP6) is essential for proper brain development. LRP6 has been shown to undergo phosphorylation at multiple PPPSP motifs upon stimulation by wingless (wnt). The phosphorylation of LRP6 by glycogen synthase kinase 3 (GSK3) and casein kinase 1 (CKI) is induced by Wnt. Regulated proteolytic processing of LRP6 has shown to release its intracellular domain (ICD). This further binds to GSK3 and inhibits its activity, resulting in activation of the canonical Wnt signaling pathway. The wnt-frizzled-LRP6 complex recruits axin to the plasma membrane. This leads to the inhibition of β-catenin phosphorylation, degradation and activation of T-cell factor/lymphoid enhancer-binding factor 1 (TCF/LEF-1)-dependent transcription. Deletion of the LRP6 gene results in severe brain abnormalities. Mutations in LRP6 are associated with coronary heart disease and osteoporosis. LRP6 is highly expressed in tumors.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.