This product is made with modified DPBS without Mg or Ca.
L9655
Linoleic Acid-Oleic Acid-Albumin, 100x
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Fatty Acid-Albumin-complexes
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352211
Form:
liquid
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biological source
protein from bovine
sterility
sterile-filtered
product line
BioReagent
form
liquid
composition
linoleic acid and oleic acid, 2 mol/mol albumin (each)
concentration
100 mg/mL BSA in DPBS
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form lyophilized powder | form viscous liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| biological source protein from bovine | biological source - | biological source bovine plasma | biological source palm oil |
| sterility sterile-filtered | sterility sterile-filtered | sterility γ-irradiated | sterility - |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
General description
Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.
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Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Laura Moody et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(2), 141-152 (2019-01-04)
Carnitine palmitoyltransferase 1 (Cpt1a) is a rate-limiting enzyme that mediates the transport of fatty acids into the mitochondria for subsequent beta-oxidation. The objective of this study was to uncover how diet mediates the transcriptional regulation of Cpt1a. Pregnant Sprague Dawley
Pierluigi Ramadori et al.
Free radical biology & medicine, 91, 114-126 (2015-12-25)
Generation of reactive oxygen species (ROS) in response to fatty acids accumulation has been classically proposed as a possible "second hit" triggering progression from simple steatosis to non-alcoholic steatohepatitis (NASH). In this study we challenged hepatocyte-specific Keap1 knockout mice (Keap1(Δhepa))
Anna Virginia Adriana Pirozzi et al.
Antioxidants (Basel, Switzerland), 10(1) (2021-01-06)
Several plant extracts are acquiring increasing value because of their antioxidant activity and hypolipidemic properties. Among them, great interest has been recently paid to açai fruit as a functional food. The aim of this study was to test the ability
Liang Tian et al.
International journal of molecular sciences, 20(5) (2019-03-13)
This study aimed to characterize the full-length cDNA of MARK4 in Sus scrofa, and evaluated its potential role in the regulation of lipid accumulation in pig placental trophoblasts and analyzed signaling pathways involved, thereby providing insights into mechanisms for placental
Jan Frohlich et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(6), 158920-158920 (2021-03-09)
Hepatocellular carcinoma (HCC) is one of the fastest-growing causes of cancer-related mortalities worldwide and this trend is mimicked by the surge of non-alcoholic fatty liver disease (NAFLD). Altered hepatic lipid metabolism promotes HCC development through inflammation and activation of oncogenes.
Articles
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Related Content
Instructions
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what kind of DPBS is used for the solution? +/- Magnesium and or Calcium?
1 answer-
Helpful?
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What is the molarity of the solution for oleic acid and linoleic acid?
1 answer-
The concentration of both Oleic and Linoleic acid in this product is 9.4 mg/L. This converts to 3.327 uM and 3.351 uM, respectively. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/239/110/l9655pis.pdfHelpful?
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