The minimum activity of this enzyme is 20 units per milligram. A survey of recent lots indicates a typical range of 45 - 50 units per milligram. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9795#product-documentation
L9795
Lactate Oxidase from Aerococcus viridans
lyophilized powder
Synonym(s):
L-lactate monooxygenase, lactate monooxygenase, lactate oxidative decarboxylase, lactate oxygenase, lactic oxidase, lactic oxygenase
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50 UNITS
$198.00
100 UNITS
$344.00
500 UNITS
$1,380.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
bacterial (Aerococcus viridans)
Quality Level
form
lyophilized powder
mol wt
80 kDa (gel filtration)
storage temp.
−20°C
1 of 4
This Item | G9888 | 59747 | G9637 |
|---|---|---|---|
| biological source bacterial (Aerococcus viridans) | biological source - | biological source - | biological source bacterial (Pediococcus spp.) |
| form lyophilized powder | form lyophilized powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 80 kDa (gel filtration) | mol wt - | mol wt - | mol wt ~76 kDa (gel filtration) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
General description
Lactate oxidase (LOX) is a flavin mononucleotide (FMN)-dependent enzyme and is a member of the α-hydroxy-acid oxidase flavoenzyme family.
Application
Lactate Oxidase from Aerococcus viridans has been used as a:
- supplement in the culture medium to convert extracellular lactate to pyruvate
- coating on glass beads for electrochemical detection of lactate from a single cell by a push-pull nozzle system
- coating on multi-branched gold nanoparticles (GNPs) for the simultaneous colorimetric detection of lactate in saliva by paper-based multiplexed device
Biochem/physiol Actions
Lactate oxidase (LOX) catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in the presence of an FMN co-factor. The enzyme aids in the determination of L-lactate indirectly by measuring the hydrogen peroxide formed in the reaction.
Other Notes
One unit is defined as the amount of enzyme which generates 1m mole of Hydrogen Peroxide per minute at 37°C under standard assay conditions.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Lin Zhou et al.
Analytical chemistry, 93(24), 8680-8686 (2021-06-10)
Metabolism of a single cell, even within the same organization, differs from other cells by orders of magnitude. Single-cell analysis provides key information for early diagnosis of cancer as well as drug screening. Any slight change in the microenvironment may
Ingar Leiros et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 12), 1185-1190 (2006-12-05)
The crystal structure of L-lactate oxidase (LOX) from Aerococcus viridans has been determined at 2.1 A resolution. LOX catalyzes the flavin mononucleotide (FMN) dependent oxidation of lactate to pyruvate and hydrogen peroxide. LOX belongs to the alpha-hydroxy-acid oxidase flavoenzyme family;
Nicholas X Williams et al.
Nature electronics, 4(4), 261-268 (2022-04-05)
Electronic waste can lead to the accumulation of environmentally and biologically toxic materials and is a growing global concern. Developments in transient electronics-in which devices are designed to disintegrate after use-have focused on increasing the biocompatibility, whereas efforts to develop
Melissa Conti Mazza et al.
Journal of neurochemistry, 162(3), 245-261 (2022-06-18)
Human DJ-1 is a cytoprotective protein whose absence causes Parkinson's disease and is also associated with other diseases. DJ-1 has an established role as a redox-regulated protein that defends against oxidative stress and mitochondrial dysfunction. Multiple studies have suggested that
Cyrielle L Bouchez et al.
STAR protocols, 3(2), 101403-101403 (2022-05-24)
Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail
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Professor Rivnay (Northwestern University, USA) discusses using organic mixed conductors as an alternative to efficiently bridge the ionic world of biology with contemporary microelectronics.
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What is the activity of this enzyme (U/g)?
1 answer-
Helpful?
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Hello, I am a research chemist. I would kindly like to know the excipients present in Lactate Oxidase from Aerococcus viridans L9795. Thank you in advance.
1 answer-
This freeze-dried preparation contains the following components:
Recombinant Lactate Oxidase at 15-20%
Sucrose at 50-84%
Potassium Phosphate monobasic at 1-30%Helpful?
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