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LH20100

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

Synonym(s):

Lipophilic Sephadex®

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Size/SKUAvailabilityPrice
50 g
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$688.00
100 g
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$1,220.00
6 x 100 g
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$4,930.00

About This Item

UNSPSC Code:
13111000
NACRES:
NB.21

$688.00


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Product Name

Sephadex® LH-20,

form

slurry

Quality Segment

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2-13

separation technique

size exclusion (SEC)

storage temp.

room temp

Application

Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc. It is also used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media.
Sephadex® LH-20 has been used to isolate bioactive polyphenolic content from blackberry genotypes[1] and tryptophan from the aqueous extract of lotus rhizomes.[2]

Legal Information

Sephadex is a registered trademark of Cytiva

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1 of 1

This Item
G50150G100120CL4B200
separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

matrix active group

polymer

matrix active group

polymer

matrix active group

polymer

matrix active group

polymer

matrix

Agarose

matrix

Agarose

matrix

Agarose

matrix

Agarose

form

slurry

form

slurry

form

powder

form

beads

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

technique(s)

LPLC: suitable

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

2-8°C


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–6 of 6 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What type of water can be used with Sephadex LH-20? Is it should be double deionized water or deionized water or distilled water?

    1 answer
    1. The manufacturer does not designate distilled or deionized water. However, a brief search of the literature reveals most publications note distilled water.

      Helpful?

  4. What solvents can be used with Sephadex LH-20, Product LH20100?

    1 answer
    1. Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents.  In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component.   Following is a table of solvents and the approximate swelling volume.  Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent                           Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide           4.4 - 4.6Pyridine                          4.2 - 4.4Water                             4.0 - 4.4Dimethyl formamide        4.0 - 4.4Methanol                        3.9 - 4.3Chloroform                     3.8 - 4.1Ethanol                           3.6 - 3.9Methylene dichloride       3.6 - 3.9Tetrahydrofuran              3.3 - 3.6Acetone                          2.4 - 2.6Acetonitrile                     2.2 - 2.4Benzene                         1.6 - 2.0Ethyl acetate                   1.6 - 1.8Toluene                          1.5 - 1.6

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is Sephadex LH-20, Product LH20100?

    1 answer
    1. Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character.  Due to its dual character, LH-20 swell in water and a number of organic solvents.  The particle size and exclusion limit vary depending on the solvent used for swelling.  The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.

      Helpful?

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