R Rossi et al.
Nucleic acids research, 25(11), 2106-2113 (1997-06-01)
ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged...
Constitutive Activation of the Nlrc4 inflammasome prevents hepatic fibrosis and promotes hepatic regeneration after partial hepatectomy
DeSantis DA, et al.
Mediators of Inflammation, 2015(2), 455-465 (2015)
ErbB2 overexpression in mammary cells upregulates VEGF through the core promoter
Loureiro RMB, et al.
Biochemical and Biophysical Research Communications, 326(2), 455-465 (2005)
I R Lehman
Science (New York, N.Y.), 186(4166), 790-797 (1974-11-29)
DNA ligase of E. coli is a polypeptide of molecular weight 75,000. The comparable T4-induced enzyme is somewhat smaller (63,000 to 68,000). Both enzymes catalyze the synthesis of phosphodiester bonds between adjacent 5'-phosphoryl and 3'-hydroxyl groups in nicked duplex DNA...
K Hayashi et al.
Nucleic acids research, 14(19), 7617-7631 (1986-10-10)
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. Similar stimulation was also caused by divalent...