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MilliporeSigma

LIG2

QuickLink™ DNA Ligation Kit

joins blunt-end and sticky-end DNA fragments

Synonym(s):

DNA Ligation Kit, Ligation Kit

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1 KIT

$539.00

$539.00

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About This Item

UNSPSC Code:
12352200
EC Number:
232-770-0
NACRES:
NA.55

Key Documents

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Molecular Biology

form

liquid

technique(s)

molecular cloning: suitable

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

usage

 kit sufficient for 50 ligation reactions

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LIG1G2N35011635379001
QuickLink™ DNA Ligation Kit joins blunt-end and sticky-end DNA fragments

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Rapid DNA Ligation Kit

Roche

11635379001

Rapid DNA Ligation Kit

technique(s)

molecular cloning: suitable

technique(s)

-

technique(s)

DNA purification: suitable

technique(s)

-

usage

 kit sufficient for 50 ligation reactions

usage

 kit sufficient for 150 ligation reactions

usage

sufficient for 350 purifications

usage

sufficient for 40 reactions (DNA ligation)

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

form

liquid

form

-

form

-

form

-

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

−70°C

storage temp.

−20°C

storage temp.

15-25°C

storage temp.

−20°C

General description

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.

Application

QuickLink™ DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence[1] and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2[2]
The QuickLink™ DNA Ligation Kit is suitable for:
  • Joining blunt or cohesive-end fragments of DNA into a cloning vector
  • Recircularization of linear DNA
  • Formation of concatamers
  • dsDNA nick repair

Biochem/physiol Actions

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

Features and Benefits

  • Fast 5 minutes ligation
  • High ligation efficiency
  • Room temperature reactions – no cooling required
  • Perform bacterial transformation with the reaction mixture

Other Notes

Sufficient for 50 reactions:
  • 500uL 2X Ligation Buffer A (L9537)
  • 100uL 5X Ligation Buffer B (L9662)
  • 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT

Legal Information

QuickLink is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000239015
0000227427
0000227427
0000239015
0000104368

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K Hayashi et al.
Nucleic acids research, 14(19), 7617-7631 (1986-10-10)
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. Similar stimulation was also caused by divalent
ErbB2 overexpression in mammary cells upregulates VEGF through the core promoter
Loureiro RMB, et al.
Biochemical and Biophysical Research Communications, 326(2), 455-465 (2005)
R Rossi et al.
Nucleic acids research, 25(11), 2106-2113 (1997-06-01)
ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged
Constitutive Activation of the Nlrc4 inflammasome prevents hepatic fibrosis and promotes hepatic regeneration after partial hepatectomy
DeSantis DA, et al.
Mediators of Inflammation, 2015(2), 455-465 (2015)
I R Lehman
Science (New York, N.Y.), 186(4166), 790-797 (1974-11-29)
DNA ligase of E. coli is a polypeptide of molecular weight 75,000. The comparable T4-induced enzyme is somewhat smaller (63,000 to 68,000). Both enzymes catalyze the synthesis of phosphodiester bonds between adjacent 5'-phosphoryl and 3'-hydroxyl groups in nicked duplex DNA
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Protocols

QuickLink™ DNA Ligation Kit

T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules

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