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LVCAS9BST

Sigma-Aldrich

CAS9 Blasticidin Lenti Particles

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NACRES:
NA.51

packaging

vial of 200 μL

Quality Level

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

selection

blasticidin

shipped in

dry ice

storage temp.

−70°C

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This Item
LVCAS9NEOCAS9BSTCAS9PROT
Cas9 Neomycin Lenti Particles

LVCAS9NEO

Cas9 Neomycin Lenti Particles

CAS9 Blasticidin Lenti Plasmid

CAS9BST

CAS9 Blasticidin Lenti Plasmid

Cas9 Protein from Streptococcus pyogenes, recombinant, expressed in E. coli, 1X NLS

CAS9PROT

Cas9 Protein

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

packaging

vial of 200 μL

packaging

vial of 200 μL

packaging

vial of 50 μL

packaging

pkg of 1 kit (3 components)

selection

blasticidin

selection

neomycin / G418

selection

ampicillin, blasticidin

selection

-

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Blasticidin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Blast using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Linkage

Click here to order gRNA plasmid and virus.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. Now complete knockout is achievable with two optimized gRNAs per gene. Minimized clone number ensures the most specific screening possible while controlling time and cost.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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